quote:
Originally posted by Wao nahele kane:
Take it up with the scientist involved in the survey.
You misunderstand me, I'm not disagreeing with anything in the article you linked to from the ncbi.
However, you also seem to misunderstand the article.
quote:
Originally posted by Wao nahele kane:
Transgenic are a source member of synthetic plasmid and in the case of the cited survey, transgenic are the source of the synthetic plasmid vector responsible for the antibiotic resistant E. coli strains.
So fyi, because your word choice makes me think you might be a little confused. Transgenic is a more accurate term for what non-scientist term 'GMO'. Genetically modified organism is a vague term. Technically it could be referring to you or me. After all we are just genetic modifications of the combination of our parents sets of chromosomes. Recombinant DNA or rDNA is also used interchangeably with transgenic. It is just a more accurate way of describing the product of moving a foreign gene from one organism to another.
So yes, synthetic plasmids are the 'cassettes' or constructs upon which the genes of interest to be moved from one organism to another are packaged inside of.
On this plasmid is what is referred to as a reporter gene, this can sometimes be an antibiotic resistance gene. Other reporter genes are used, however, besides antibiotic resistance genes. Another example of a reporter gene is the GFP gene from jellyfish. These genes have different uses and it would not always be appropriate to use one or the other depending on the nature of the organism you are trying to improve and how you wish to observe he presence of the gene of interest.
So to recap a plasmid is a small circular extra chromosomal piece of DNA that we can manipulate to include genes of interest think herbicide tolerance or insect tolerance. As well as the gene of interest there is also the reporter gene like antibiotic resistance or GFP. Everything within a specific region of the plasmid is transferred over into the target organism. Knowing this enables us to know that if the reoporter gene made it into the organism then the gene of interest made it as well.
quote:
Originally posted by Wao nahele kane:
But what the heck, empirical evidence be damned because it doesn't fit your rational paradigm. Nice.
My paradigm on this matter starts and ends in molecular biology. Please consult molecular cloning: a laboratory manual by Tom Maniatus for further information about plasmids.
quote:
Originally posted by Wao nahele kane:
You can now firmly welcome this antibiotic resistant E.coli bacteria or one like it with open arms when it ends up in your fast food burger.
I don't know where you came up with this idea but if you really think that based off the paper you read on the ncbi you will soon have antibiotic resistant E.coli in your burger you should probably take a basic biology course.
quote:
Originally posted by Wao nahele kane:
Why? Because you're a GMO proponent and welcome all things influenced by GMO because its 100% safe in your opinion.
I've actually said many times on punaweb nothing is 100% safe. Literally. Name one thing thats 100% safe. You can't! I don't think transgenic foods are any more or less safe that conventional, fwiw.
quote:
Originally posted by Wao nahele kane:
Forget the fact that no research is required to test the influence these products have on the world of the countless micro biologic organisms that surround us.
I don't know why you think this but in reality corporations have to go through an extensive battery of testing to the tune of severa hundred million dollars per crop just to get their perfectly safe products licensed to be produced commercially. It's this extensive R&D investment that necessitates their patenting of intellectual property rights.
Oh and back to plasmids and reporter genes. They will soon be replaced by recent technology which is easier to manipulate and more efficient.
New technologies have since come into use which are making this technique insignificant. It's now much easier to make genome modifications without the need to insert DNA from a foreign host. Small molecular machines called CAS proteins are now utilized to make insertion and deletions at specific loci. This new technology is driven by gRNA to the site of interest and has applications that are widespread. A researcher in China has induced resistance to powdery mildew in wheat using these new methods and current research is ongoing to inhibit Mosquitoes ability to transmit malaria and dengue fever. Future applications in material science like biomaterials that can absorb co2 (a greenhouse gas) from the atmosphere making 'super green' homes.
So. I wouldn't be worried about 'GMOs' causing a catastrophe via antibiotic resistance. Antibiotic resistance is a real problem and should be addressed seriously. However, the role of resistance and mutation is a well studied and understood biological phenomena and is completely separate of 'GMOs'. Bactetia will evolve further resistance in the future but it will be because of a natural process that has been happening for millions of years if not billions and not because of 'GMOs'.
just my .02 cents
Hope you learned something!